The first time, not written. Directly to show the original file to everyone !! The principle is to use JS over all Checkbox to set all the Checkbox unchecked, and then set the clicked checkbox to select the following below. ! ASPX file <% @ Page language = "c #" Codebehind = "WebForm1.aspx.cs" AutoEventWireup = "false" Inherits = "DataGridCheck.WebForm1"%> Webform1 title>